TABLE 1.

Phenotypic and genotypic characterization of isolates^a

Origin of strain	Strain no.	Sample type	Serotypeb	Hemolysin phenotypec	Status regarding:					stx2 variant(s)
					eae gene	ehxA gene	Verocytotoxin production	stx1 gene	stx2 gene
Wastewater treatment plant	V1	Slurry	O166:H28	ehly	N	P	P	N	P	stx2e, stx2d-Ount
	V2	Clarifier	O113:H4	N	P	P	P	N	P	stx2-NV206, stx2d
	V3	Slurry pit	O2:H27	N	N	P	P	N	P	stx2-EDL933, stx2d
	V4	Aerator	O76:H19	ehly	P	P	P	P	P	stx2e, stx2d-Ount
	V5	Clarifier	O26:H11	ehly	P	P	P	P	N	N
	V6	Sludge	O76:H19	ehly	N	P	P	P	P	stx2e, stx2vh-a, stx2d-Ount
	V7	Clarifier	O76:H19	ehly	P	P	P	P	P	stx2e, stx2vh-a, stx2d-Ount
	V8		O76:H19	ehly	N	P	P	N	P	stx2-EDL933
	V9	Aerator	O157:H7	ehly	P	P	P	P	P	stx2vh-a, stx2d-Ount
	V10	Clarifier	O11:H43	N	N	N	P	N	P	N
Pig farms	V11	Feces	Ont:H4	alpha	N	N	P	N	P	stx2e
	V12	Feces	O141:H4	alpha	N	N	P	N	P	stx2e
	V13	Feces	O8:H19	−N	N	N	P	N	P	stx2-NV206, stx2e, stx2d
	V14	Feces	O103:H2	ehly	P	P	P	N	P	stx2e, stx2d
	V15	Feces	Ont:H19	N	N	P	P	P	P	stx2vh-b, stx2e, stx2d-Ount
	V16	Feces	O121:H−	N	N	N	P	N	P	stx2e
Dairy cattle herd	V17	Feces	Ont:H38	N	P	P	P	N	P	stx2vh-a, stx2d-Ount
	V18	Feces	Orough:H25	ehly	P	P	P	N	P	stx2e, stx2vh-a, stx2d-Ount
	V19	Feces	Orough:H25	alpha	P	P	P	N	P	stx2vh-a, stx2d-Ount
	V20	Feces	O113:H4	N	N	N	P	N	P	stx2-NV206, stx2e, stx2d
	V21	Manure	O174:H21	N	N	N	P	N	P	stx2vh-b, stx2e, stx2d-Ount
	V22	Manure	O174:H21	N	N	N	P	N	P	stx2vh-b, stx2e, stx2d-Ount
	V23	Manure	O174:H21	ehly	N	P	P	P	P	stx2e, stx2-EDL933, stx2d
	V24	Slurry pit	O8:H19	ehly	N	P	P	P	N	N
	V25		Ont:H8	N	N	P	P	P	N	N
	V26	Feces	O174:H−	N	N	N	P	N	P	stx2vh-a, stx2d-Ount
	V27	Manure	O8:H19	ehly	N	P	P	P	P	stx2-EDL933, stx2d

^aP, positive; N, negative.

^bOrough, rough; Ont, O type not corresponding to any serogroup between O1 and O174.

^cehly, hemolysis after a 22-h incubation step; alpha, hemolysis after 3- to 4-h incubation step.