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Effect of mutations in several sulfur metabolism genes on resistance to acetaldehyde. Five-microliter aliquots of serial dilutions from exponential cultures in YPD medium (2% [wt/vol] glucose) were spotted onto YPD or YPD plus acetaldehyde (1 g/liter) plates. Plates were incubated for several days until colonies appeared. Experiments were performed at least three times. The figure shows the results of one representative experiment.
