FIG.2.

Phylogenetic tree for genus Pseudoalteromonas based on almost complete 16S rRNA gene sequences. The maximum parsimony analysis was performed with standard settings in ARB. Phylogenetic affiliations of organisms represented by partial 16S rRNA gene sequences were reconstructed by applying parsimony criteria without changing the overall tree topology. DIRECT, sequences obtained via direct sequencing; DGGE, sequences obtained via DGGE bands cut out from the gel; CLONE, sequences obtained via cloning. DGGE-P. tunicata was a DGGE band cut from a pure culture of P. tunicata as a control. Accession numbers are in brackets. Underlined strains have been reported to be associated with eukaryotes. The asterisk indicates the subgroup containing the antifouling bacteria P. tunicata and P. ulvae.