Fig. 1.

Expression of the components and activity of Wnt signaling during SC development. Analysis of the expression of canonical Wnt signaling components (A–F) by in situ hybridization at two different stages of SC development (in each panel, Upper shows dorsal root ganglia, and Lower shows nerves) and (G) by qRT-PCR in primary SC cultures. Canonical Wnt signaling is synergistically activated by Wnt and Rspondin ligands in cultured SCs. (H) Dose-dependent coactivation of canonical Wnt signals by Wnt3a and Rspo3, determined by increase in the expression of the classical Wnt target gene Axin2. (I) Endogenous expression of Axin2 in mouse sciatic nerves measured by qRT-PCR is highest at E15.5 and decreases postnatally. Error bars represent SD (n = 5); the value at E15.5 is from embryonic sciatic nerves from 10 embryos pooled as a single probe. AU, arbitrary units. (Scale bars: 100 μm.)