Fig. 4.

Identification of an ALX/FPR1 signaling signature. (A) Proteome profiler array on HEK293 cells transfected with FPR1, ALX, or both FPR1 and ALX, before 10-min stimulation with Ac2-26 (10⁻⁵ M). Control cells were left unstimulated. (B) Western blotting analysis on HEK293 cells (1 × 10⁶) transfected with human FPR1, ALX, or FPR1 + ALX to assess the phosphorylation status of ERK and JNK (Top and Middle). Jnk phosphorylation in neutrophils stimulated with Ac-26 for 10 min (Bottom). Blots are representative of three individual experiments.