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. 2013 Oct 9;110(45):18232–18237. doi: 10.1073/pnas.1308253110

Fig. 5.

Fig. 5.

Peptide Ac2-26 rescues the antiapoptotic effect of SAA. (A and B) Human neutrophils (1 × 106) were incubated with SAA (10 μg/mL) alone or with Ac2-26 (10−5 M), before assessment of early (6–12 h) and late (24 h) apoptosis by flow cytometry (AnxAV- FITC/PI double staining). Experiments were run in the absence (A) or presence (B) of the JNK inhibitor SP600125 (2 × 10−6 M) added to cells 30 min before. Data are mean ± SEM, n = three independent donors. *P < 0.05 one-way ANOVA (Ac2-26 vs. 0 concentration); #P < 0.05 two-way ANOVA (vehicle vs. SAA). (C) Western blotting for caspase-3 cleavage in human neutrophils treated with SAA alone or together with Ac2-26 (10−5 M) for 24 h in the presence or absence of SP600125 (2 × 10−6 M). Blot representative of three experiments.