Fig. 1.

HSV-1 induces MT stabilization in primary human fibroblasts. (A–C) NHDFs were mock-infected or infected with HSV-1 strain F at m.o.i. 20. (A) Samples were fixed at the indicated times and stained for Tyr-MTs (red) and Ac-MTs (green). Arrows highlight representative perinuclear dots of Tyr-tubulin at the centrosome. (B and C) Infected samples fixed at 9 h.p.i. were stained for (B) Tyr-MTs (red) and EB1 (green) or (C) Ac-MTs (green) and EB1 (red). Magnification 100×. Arrows point to Tyr-MT ends with EB1 comets (B) or Ac-MTs lacking EB1 (C). (D) NHDFs or HeLa cells were infected at m.o.i. 2 for 16 h with the indicated HSV-1 strains, and lysates were analyzed by WB using the indicated antibodies. Ac-Tub, acetylated tubulin; T-Tub, total tubulin. The arrow points to GFP-tagged Us11 in Patton strain HSV-1-GFP-Us11 (Patt-Us11).