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. 2013 Oct 21;110(45):18268–18273. doi: 10.1073/pnas.1310760110

Fig. 4.

Fig. 4.

The TGN acts as an alternate MTOC in HSV-1-infected cells. NHDFs were mock-infected or infected with HSV-1 at m.o.i. 5 for 14 h. Cultures were treated for 3 h with DMSO or 10 μM nocodazole. Nocodazole was then washed out (w/o) for the indicated times. (A) Fixed samples were stained for Tyr-MTs (red) and TGN46 (green). (B) Higher-magnification images of samples from A illustrate Tyr-MT growth from TGN structures, highlighted by arrows. (C) Fixed samples were stained for TGN46 (red) and Ac-MTs (green). (D) Higher magnification of samples 15 min after w/o in B. Arrows point to Ac-MTs radiating from TGN46-positive structures.