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. 2013 Oct 21;110(45):18168–18173. doi: 10.1073/pnas.1311382110

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Fig. 4. — Cotransfection of EloB/C does not affect VHL protein decrease. (A) HeLa cells were cotransfected with a panel of SOCS domain proteins and their respective BC-box mutant proteins. EloB/C were cotransfected as indicated (+ or −). Lysates were probed against VHL, EloB/C, and SAE1. Loading control: Actin. Decrease in SAE1 is specific to Gam1 WT protein expression and is the positive control. (B) Graph of VHL protein levels (from immunoblot in A) based on pixel-intensity values measured from plot profiles of individual lanes using ImageJ. (C–E) Immunoblots of transfected proteins with loading controls.