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. 2013 Oct 21;110(45):18168–18173. doi: 10.1073/pnas.1311382110

Fig. 5.

Fig. 5.

Cullin2 and Cullin5 are involved in VHL decrease. (A) HeLa cells were transfected with shCul2 and shCul5 or control vector. VHL levels relative to Actin loading show a less significant decrease in VHL upon Cul2 and Cul5 knockdown in SOCS domain-transfected cells. (B) Transient expression of the transfected proteins is shown by IB. (C) HeLa cells selected as described in A were transfected as depicted. Flag Ub was IP under SDS lysis conditions (SI Materials and Methods). Loading control: Tubulin.