Table 1.
Primer sequences, PCR product sizes, and references for enzymatic mismatch cleavage
| Number | Primer name | Primer sequence | Product size (bp) | Source |
|---|---|---|---|---|
| 1 |
rdg2a_F1 |
CTTGCTCTCAAGACAATGGGTGGATTG |
1499 |
1 |
| |
rdg2a_R1 |
TCCAAACTGCTAAACATCCGAGGCTCT |
|
|
| 2 |
rdg2a_F2 |
CTCTCAAGACAATGGGTGGATTGCTGA |
1493 |
1 |
| |
rdg2a_R2 |
CAAACTGCTAAACATCCGAGGCTCTCC |
|
|
| 3 |
rdg2a_F3 |
TCGCTATGTCAAGAGCTGGATGAAGGA |
1496 |
1 |
| |
rdg2a_R3 |
AAGTGCGTAGGATTGTTCTGCCTTTGC |
|
|
| 4 |
nbs2-rdg2a_F1 |
GCTCTTCCGTTTTGAAATGAGCAGGAA |
1503 |
1 |
| |
nbs2-rdg2a_R1 |
TGTTTTGCATTTATGGCCTTGCAAATG |
|
|
| 5 |
nbs2-rdg2a_F2 |
TCCACTACCCGAAAGGCACTCAGCTAC |
1500 |
1 |
| |
nb2-rdg2a_R2 |
GCAATGCAATGCTCTTACTGACGCAAA |
|
|
| 6 |
nb2-rdg2a_F3 |
TCGAACGAATCAGTGGGTTATGCAAAG |
1497 |
1 |
| |
nb2-rdg2a_R3 |
ATGAAGTGTTCCCCTCCAGGTTGTCAC |
|
|
| 7 |
nbs3-rdg2a_F1 |
TGGCAAGTCCACTACCAAAAAGGCACT |
1491 |
1 |
| |
nbs3-rdg2a_R1 |
GCTCTTAGTGATGCCAATACCCGTTGC |
|
|
| 8 |
nbs3-rdg2a_F2 |
GCTCTTCCGTTTTGAAATGAGCAGGAA |
1491 |
1 |
| |
nbs3-rdg2a_R2 |
TGTTTTGCATTTATGGCCTTGCAAATG |
|
|
| 9 |
HVgna1f |
GACCCAGATGGCATCCAC |
552 |
2 |
| |
HVgna1r |
ATGCGACGAGACAAAGGAAT |
|
|
| 10 |
HVraa1f |
GTCGACGACTTGCATCATCTATCG |
545 |
2 |
| |
HVraa1R |
CACCCCGATCACTAACACACAA |
|
|
| 11 |
HV_hpa1F |
CCCTTATGTGTACCCTGATCCTGA |
1100 |
2 |
| |
HV_hpa1R |
GGTCCAACAGACGTATTAGCCAAG |
|
|
| 12 |
HV_Mlo9-F1 |
AGCAAACCAGACACACAGCAGCGTACC |
900 |
3 |
| |
HV_Mlo9-R1 |
GCAAAGGCTCACTTTGAGACGGCTTAG |
|
|
| 13 |
HV_Mlo9-F2 |
CATTTGTCGCAAAACAGCAAGTTCGAC |
1476 |
3 |
| |
HV_Mlo9-R2 |
TTGTCTCATCCCTGGCTGAAGGAAAAA |
|
|
| 14 |
HvHox1-F1 |
AAGCATGGACAAGCATCAGCTCTTTGA |
985 |
3 |
| |
HvHox1-R1 |
GGCAGCAGCTATCTCGGCTATTTTATGG |
|
|
| 15 |
HV_Kap1_c1_F |
TCCACCGGTAAAGAAACCAG |
1030 |
4 |
| |
HV_Kap1_c1_R |
TGAGGGAGGGAGAAAGATGA |
|
|
| 16 |
HV_Kap1_d1_F |
CTCCCTCCCTCAAGAAATCC |
899 |
4 |
| |
HV_Kap1_d1_R |
GCTGTCGCAAAATACAGCAA |
|
|
| 17 |
HV_Kap1_e1_F |
TTGCTGTATTTTGCGACAGC |
1136 |
4 |
| |
HV_Kap1_e1_R |
CATGTGTTAAAAGCCGCAGA |
|
|
| 18 |
HV_Kap1_g1_F |
ATTGAGTGCCTCTCGGCTTA |
1175 |
4 |
| |
HV_Kap1_g1_R |
TGAGGAAAGAAGGGATGTGG |
|
|
| 19 |
HV_Kap1_h1_F |
CCACATCCCTTCTTTCCTCA |
718 |
4 |
| |
HV_Kap1_h1_R |
GGGAGCTTGCCTTTCTTCTT |
|
|
| 20 |
HV_Kap1_i1_F |
TGTGGAACTATAAATCTGGCTTCA |
698 |
4 |
| |
HV_Kap1_i1_R |
CGAGCTAGCCGAACCTGTAG |
|
|
| 21 |
Hv_mloA-F |
CGTGTGCGTACCTGGTAGAG |
599 |
5 |
| |
Hv_mloA-R |
CAAGCCAAGACGACAATCAG |
|
|
| 22 |
Hv_mloB-F |
CTGATTGTCGTCTTGGCTTG |
624 |
5 |
| |
Hv_mloB-R |
CTGACTCCATACGCCAAACA |
|
|
| 23 |
Hv_mloC-F |
TGTTTGGCGTATGGAGTCAG |
566 |
5 |
| |
Hv_mloC-R |
AGAAACCGGAGAGGAGAAGG |
|
|
| 24 |
Hv_mloD-F |
CCTCACCCTCTTCCTTGACA |
574 |
5 |
| |
Hv_mloD-R |
CGTCAGAGCAGTTCATCAGC |
|
|
| 25 |
Hv_mloE-F |
CCACCGATGAACTTGTCAGT |
837 |
5 |
| |
Hv_mloE-R |
GAGAGGGGTTTTGTTTGTGC |
|
|
| 26 |
Hv_Mla1-3 F |
AGCAGCTCGACAGCCAAGACAA |
451 |
5 |
| Hv_Mla1-3R | CCCAACCCTCCAAATCCAACAA |