Table 3.
Summary of SSR primers tested, quality of PCR products and polymorphisms detected
|
Primer pair
1
|
PCR amplification
2
|
Polymorphisms detected between parents |
|
|---|---|---|---|
| GP | HOR1606 | GP + HOR1606 | |
| cnl34 |
W |
W |
N |
| cnl73 |
? |
? |
N |
| cnl31 |
Y |
Y |
N |
| cnl146 |
Y |
Y |
Y |
| cnl130 |
Y |
Y |
? |
| cnl151 |
N |
Y |
--- |
| cnl140 |
N |
N |
--- |
| HVACL1 |
Y |
Y |
? |
| HVLEU |
Y |
Y |
N |
| HVGNIRE |
Y |
Y |
N |
| HVCSG |
N |
N |
--- |
| HVADH1 |
N |
N |
--- |
| HVWAXYG |
? |
? |
N |
| HVDHN7 |
Y |
? |
? |
| HVCMA |
Y |
Y |
Y |
| HVDHN9 |
Y |
Y |
N |
| HVBKASI |
Y |
W |
? |
| HVPRP1B |
? |
Y |
? |
| HVBDG |
W |
W |
N |
| HVBARE1 |
N |
N |
--- |
| HVRCABG |
W |
N |
--- |
| HVSIP1A |
Y |
Y |
N |
| Bmac0113 |
Y |
Y |
Y |
| Bmag0323 |
Y |
Y |
N |
| Bmac0812 |
Y |
Y |
N |
| Bmag0477 |
W |
W |
? |
| Bmag0387 |
Y |
W |
? |
| Bmac0163 |
Y |
Y |
N |
| GBM1284 |
? |
? |
--- |
| Bmag0346 |
N |
N |
--- |
| Bmag0023 |
N |
N |
--- |
| GBM1300 | Y | Y | N |
1From http://germinate.scri.ac.uk/ssr/barley_s.html.
2Y = yes, W = weak amplification, N = no, ? = unclear results, --- = not tested.