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. Author manuscript; available in PMC: 2014 Aug 20.
Published in final edited form as: Biochemistry. 2013 Jul 31;52(33):10.1021/bi400556e. doi: 10.1021/bi400556e

Fig. 1. Phenotypes of MRDI and MtnA expression and deletion.

Fig. 1

(A) Growth in full media of A375 melanoma cells, carrying empty vector (Vector), depleted of MRDI with shRNA (KD), depleted of endogenous MRDI and expressing a wild-type bypass MRDI (bpWT), or depleted of endogenous MRDI and expressing B. subtilis MtnA (MtnA). The bars represent cell counts for time 0, 48 and 96 hours normalized to that of cells carrying empty vector at time = 0h. (B) Same as (A) but with cells grown in methionine-free media supplemented with 30 μM MTA. (C) Quantitation of cell invasiveness in 3D spheroid assays carried as described in Materials and Methods. Values represent the mean of 10-11 independent experiments. The error bars represent standard deviation of the mean. (D) Representative images of 3D spheroid invasion assays, for cells quantified in (C).