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. 2013 May 31;2(4):553–565. doi: 10.1002/mbo3.93

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Schematic representation of the pET-P_T7(RpoD), pET-P_T7(FliA), pACYC-P_T7(GFP), pACYC-P_rrnB(GFP), and pACYC-P_rrnB(Luc) plasmids. Ori, Escherichia coli replication origin; Kan^r and Amp^r, genes conferring resistance to kanamycin and ampicillin, respectively; RpoD, OY-M rpoD gene without its own promoter; FliA, OY-M fliA gene without its own promoter; LacI, lactose repressor; P_T7/lacI, T7 promoter regulated by LacI; P_rrnB, transcription promoter of the OY-M rrnB gene; GFP,gfp reporter gene; Luc, luciferase reporter gene; N, NdeI; S, SalI; B, BglII; H, HindIII.