Plasmids used in this study
Plasmid | Description | Source |
---|---|---|
pET-30a | A expression vector with lac-inducible T7 promoter; ColE1; Kanr | Novagen |
pET-RpoD | pET-30a carrying a OY phytoplasmal rpoD (PAM628); Kanr | This study |
pET-FliA | pET-30a carrying a OY phytoplasmal fliA (PAM320); Kanr | This study |
pACYC177 | A low-copy-number plasmid; p15A; Ampr; Kanr | Fermentas |
pACYC-PrrnB (GFP) | A promoter-probe vector containing Δkan::gfp with promoter region of OY phytoplasmal rrnB (PAM_r006) promoter; Ampr | This study |
pACYC-PrrnB (Luc) | A promoter-probe vector containing Δkan::luciferase with promoter region of OY phytoplasmal rrnB (PAM_r006) promoter; Ampr | This study |
pACYC-PrpsJ (Luc) | A promoter-probe vector containing Δkan::luciferase with promoter region of OY phytoplasmal rpsJ (PAM199) promoter; Ampr | This study |
pACYC-PgyrB (Luc) | A promoter-probe vector containing Δkan::luciferase with promoter region of OY phytoplasmal gyrB (PAM498) promoter; Ampr | This study |
pACYC-P289 (Luc) | A promoter-probe vector containing Δkan::luciferase with promoter region of OY phytoplasmal PAM289 promoter; Ampr | This study |
pACYC-PmdlB (Luc) | A promoter-probe vector containing Δkan::luciferase with promoter region of OY phytoplasmal mdlB (PAM059) promoter; Ampr | This study |
pACYC-Ptengu (Luc) | A promoter-probe vector containing Δkan::luciferase with promoter region of OY phytoplasmal tengu (PAM765) promoter; Ampr | This study |
pACYC-PhflB (Luc) | A promoter-probe vector containing Δkan::luciferase with promoter region of OY phytoplasmal hflB (PAM064) promoter; Ampr | This study |
pACYC-PhimA (Luc) | A promoter-probe vector containing Δkan::luciferase with promoter region of OY phytoplasmal himA (PAM317) promoter; Ampr | This study |
pACYC-Pdam (Luc) | A promoter-probe vector containing Δkan::luciferase with promoter region of OY phytoplasmal dam (PAM565) promoter; Ampr | This study |