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. 2013 May 31;2(4):553–565. doi: 10.1002/mbo3.93

Plasmids used in this study

Plasmid Description Source
pET-30a A expression vector with lac-inducible T7 promoter; ColE1; Kanr Novagen
pET-RpoD pET-30a carrying a OY phytoplasmal rpoD (PAM628); Kanr This study
pET-FliA pET-30a carrying a OY phytoplasmal fliA (PAM320); Kanr This study
pACYC177 A low-copy-number plasmid; p15A; Ampr; Kanr Fermentas
pACYC-PrrnB (GFP) A promoter-probe vector containing Δkan::gfp with promoter region of OY phytoplasmal rrnB (PAM_r006) promoter; Ampr This study
pACYC-PrrnB (Luc) A promoter-probe vector containing Δkan::luciferase with promoter region of OY phytoplasmal rrnB (PAM_r006) promoter; Ampr This study
pACYC-PrpsJ (Luc) A promoter-probe vector containing Δkan::luciferase with promoter region of OY phytoplasmal rpsJ (PAM199) promoter; Ampr This study
pACYC-PgyrB (Luc) A promoter-probe vector containing Δkan::luciferase with promoter region of OY phytoplasmal gyrB (PAM498) promoter; Ampr This study
pACYC-P289 (Luc) A promoter-probe vector containing Δkan::luciferase with promoter region of OY phytoplasmal PAM289 promoter; Ampr This study
pACYC-PmdlB (Luc) A promoter-probe vector containing Δkan::luciferase with promoter region of OY phytoplasmal mdlB (PAM059) promoter; Ampr This study
pACYC-Ptengu (Luc) A promoter-probe vector containing Δkan::luciferase with promoter region of OY phytoplasmal tengu (PAM765) promoter; Ampr This study
pACYC-PhflB (Luc) A promoter-probe vector containing Δkan::luciferase with promoter region of OY phytoplasmal hflB (PAM064) promoter; Ampr This study
pACYC-PhimA (Luc) A promoter-probe vector containing Δkan::luciferase with promoter region of OY phytoplasmal himA (PAM317) promoter; Ampr This study
pACYC-Pdam (Luc) A promoter-probe vector containing Δkan::luciferase with promoter region of OY phytoplasmal dam (PAM565) promoter; Ampr This study