Figure 1.

The amount of EC-12 phagocytosed by peritoneal macrophages from WT, TLR2-, TLR7-, and MyD88-KO mice. The peritoneal macrophages preincubated in a 96-well microplate were cocultured with FITC-labeled EC-12 for 1 (A) or 2 h (B). After nuclear staining with DAPI, the levels of DAPI and FITC fluorescence were measured using a microplate fluorometer. Measurements of FITC fluorescence were normalized to DAPI levels. The normalized value was centuplicated and expressed as arbitrary units (AUs). Error bars indicate the standard deviation of the mean. WT, wild-type; TLR, toll-like receptor; KO, knockout; FITC, fluorescein isothiocyanate; DAPI, 4′, 6-diamidino-2-phenylindole.