Bacteria and plasmids
| Strains | Relevant characteristics1 | Source |
|---|---|---|
| Escherichia coli | ||
| W3110 | F− IN(rrnD-rrnE)1 | Hill and Harnish (1981) |
| RA1027 | W3110-ΔtolQ | Present study |
| RA1028 | W3110-ΔtolR | Present study |
| RA1038 | W3110-ΔtolA | Weitzel and Larsen (2008) |
| BacterioMatch II reporter | hisB lac [F′ lacIq HIS3 aadA Kmr] | Stratagene2 |
| Enterobacter amnigenus | ||
| (ATCC51816) | Clinical isolate | ATCC3 |
| Cronobacter muytjensii | ||
| (ATCC51329) | Clinical isolate | ATCC3 |
| Plasmids | ||
| pBAD18-Cm | araBAD promoter, AraC, cmr | Guzman et al. (1995) |
| pBAD24 | araBAD promoter, AraC, ampr | Guzman et al. (1995) |
| pKP315 | pBAD24 encoding TonB | Larsen et al. (1999) |
| pKP660 | pBAD24 encoding ExbBD | Ollis and Postle (2012) |
| pBT | lambda cI fusion site, cmr | Stratagene |
| pBT-LGF2 | lambda cI-LGF2 fusion, cmr | Stratagene |
| pTRG | RNAPα fusion site, tetr | Stratagene |
| pTRG-Gal11P | RNAPα-Gal11P fusion, tetr | Stratagene |
| pRA002 | pBAD24 encoding TolR | Brinkman and Larsen (2008) |
| pRA003 | pBAD24 encoding TolQR | Brinkman and Larsen (2008) |
| pRA004 | pBAD24 encoding TolA | Weitzel and Larsen (2008) |
| pRA031 | pBAD24 encoding TolQ | Present study |
| pMT001 | pBAD24 encoding FtsN | Present study |
| pMT002 | pBAD18-Cm encoding TolQ | Present study |
| pMT005 | pBT lambda cI – TolQ(1-19) | Present study |
| pMT006 | pBT lambda cI – TolQ(39-135) | Present study |
| pMT007 | pBT lambda cI – TolQ(157-174) | Present study |
| pMT008 | pBT lambda cI – TolQ(194-230) | Present study |
| pMT009 | pTRG RNAPα – FtsN(1-33) | Present study |
| pMT010 | pTRG RNAPα – FtsN(54-243) | Present study |
| pMT011 | pTRG RNAPα – FtsN(54-319) | Present study |
1
The specific nature of the deletions (∆) is as follows: The ∆tolQ deletion removed the predicted tolQ codons 1-230 and the termination codon; the ∆tolR deletion removed the predicted tolR codons 1-142 and the termination codon; the ∆tolA deletion removed the predicted tolA codons 1-421, leaving the termination codon in place.
2
Purchased from Stratagene.
3
Purchased from the American Type Culture Collection (ATCC).