Table 5.
Susceptibility of V. parahaemolyticus RND-type efflux transporter deficient mutants to various antimicrobial agents
| MIC (μg mL−1) | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Strain | Deleted genes1 | OXA | CLO | EM | NFLX | NOV | TET | KM | CM | BC | CHL | EtBr | R6G | ACR | TPP | HOE | SDS | CHO | DEO | GLY |
| AQ3334 | parental strain | 128 | 128 | 4 | 0.06 | 8 | 1 | 16 | 0.5 | 16 | 32 | 128 | 128 | 16 | 128 | 2 | 51,200 | >32,000 | 8000 | >32,000 |
| TM3 | AB | 64 | 128 | 4 | 0.06 | 8 | 1 | 16 | 0.5 | 16 | 32 | 64 | 128 | 8 | 128 | 0.5 | 51,200 | >32,000 | 8000 | >32,000 |
| TM4 | CD | 64 | 32 | 2 | 0.06 | 8 | 1 | 16 | 0.5 | 16 | 16 | 128 | 16 | 16 | 64 | 2 | 1600 | 4000 | 2000 | >32,000 |
| TM5 | EF | 128 | 128 | 4 | 0.06 | 8 | 1 | 16 | 0.5 | 16 | 32 | 128 | 128 | 16 | 128 | 2 | 51,200 | >32,000 | 8000 | >32,000 |
| TM6 | HI | 128 | 128 | 4 | 0.06 | 8 | 1 | 16 | 0.5 | 16 | 32 | 128 | 128 | 16 | 128 | 2 | 51,200 | >32,000 | 8000 | >32,000 |
| TM7 | K | 128 | 128 | 4 | 0.06 | 8 | 1 | 16 | 0.5 | 16 | 32 | 128 | 128 | 16 | 128 | 2 | 51,200 | >32,000 | 8000 | >32,000 |
| TM8 | LM | 128 | 128 | 4 | 0.06 | 8 | 1 | 16 | 0.5 | 16 | 32 | 128 | 128 | 16 | 128 | 2 | 51,200 | >32,000 | 8000 | >32,000 |
| TM9 | O | 128 | 128 | 4 | 0.06 | 8 | 1 | 16 | 0.5 | 16 | 32 | 128 | 128 | 16 | 128 | 2 | 51,200 | >32,000 | 8000 | >32,000 |
| TM10 | Q | 128 | 128 | 4 | 0.06 | 8 | 1 | 16 | 0.5 | 16 | 32 | 128 | 128 | 16 | 128 | 2 | 51,200 | >32,000 | 8000 | >32,000 |
| TM11 | RS | 128 | 128 | 4 | 0.06 | 8 | 1 | 16 | 0.5 | 16 | 32 | 128 | 128 | 16 | 128 | 2 | 51,200 | >32,000 | 8000 | >32,000 |
| TM12 | UV | 128 | 128 | 4 | 0.06 | 8 | 1 | 16 | 0.5 | 16 | 32 | 128 | 128 | 16 | 128 | 2 | 51,200 | >32,000 | 8000 | >32,000 |
| TM13 | WX | 128 | 128 | 4 | 0.06 | 8 | 1 | 16 | 0.5 | 16 | 32 | 128 | 128 | 16 | 128 | 2 | 51,200 | >32,000 | 8000 | >32,000 |
| TM14 | YZ | 128 | 128 | 4 | 0.06 | 8 | 1 | 16 | 0.5 | 16 | 32 | 128 | 128 | 16 | 128 | 2 | 51,200 | >32,000 | 8000 | >32,000 |
| TM32 | AB, CD | 32 | 8 | 0.25 | 0.03 | 2 | 1 | 16 | 0.5 | 8 | 16 | 8 | 8 | 8 | 16 | 0.5 | 400 | 2000 | 1000 | >32,000 |
| TM33 | AB, CD, EF | 16 | 8 | 0.25 | 0.03 | 0.5 | 1 | 16 | 0.5 | 8 | 16 | 4 | 8 | 8 | 16 | 0.5 | 200 | 1000 | 1000 | >32,000 |
| TM34 | AB, CD, EF, YZ | 16 | 2 | 0.25 | 0.03 | 0.5 | 1 | 16 | 0.5 | 8 | 8 | 4 | 8 | 8 | 16 | 0.5 | 200 | 1000 | 1000 | 1000 |
| TM35 | AB, CD, EF, YZ, K | 16 | 2 | 0.25 | 0.03 | 0.25 | 1 | 16 | 0.5 | 8 | 8 | 4 | 8 | 8 | 16 | 0.5 | 200 | 1000 | 1000 | 1000 |
| TM36 | AB, CD, EF, YZ, K, HI | 16 | 2 | 0.25 | 0.03 | 0.25 | 1 | 16 | 0.5 | 8 | 8 | 4 | 8 | 8 | 16 | 0.5 | 100 | 1000 | 500 | 1000 |
| TM37 | AB, CD, EF, YZ, K, HI, LM | 16 | 2 | 0.25 | 0.03 | 0.25 | 1 | 16 | 0.5 | 4 | 4 | 4 | 2 | 8 | 16 | 0.5 | 50 | 500 | 125 | 1000 |
| TM38 | AB, CD, EF, YZ, K, HI, LM, UV | 16 | 2 | 0.25 | 0.03 | 0.25 | 1 | 16 | 0.5 | 4 | 4 | 4 | 2 | 8 | 16 | 0.5 | 50 | 500 | 125 | 1000 |
| TM39 | AB, CD, EF, YZ, K, HI, LM, UV, WX | 16 | 2 | 0.25 | 0.03 | 0.25 | 1 | 16 | 0.5 | 4 | 4 | 4 | 2 | 8 | 16 | 0.5 | 50 | 500 | 125 | 1000 |
| TM310 | AB, CD, EF, YZ, K, HI, LM, UV, WX, Q | 16 | 2 | 0.25 | 0.03 | 0.25 | 1 | 16 | 0.5 | 4 | 4 | 4 | 2 | 8 | 16 | 0.5 | 50 | 500 | 125 | 1000 |
| TM311 | AB, CD, EF, YZ, K, HI, LM, UV, WX, Q, RS | 16 | 2 | 0.25 | 0.03 | 0.25 | 1 | 16 | 0.5 | 4 | 4 | 4 | 2 | 8 | 16 | 0.5 | 50 | 500 | 125 | 1000 |
| TM312 | AB, CD, EF, YZ, K, HI, LM, UV, WX, Q, RS, O | 16 | 2 | 0.25 | 0.03 | 0.25 | 1 | 16 | 0.5 | 4 | 4 | 4 | 2 | 8 | 16 | 0.5 | 50 | 500 | 125 | 1000 |
| TM15 | vpoC | 4 | 1 | 0.25 | 0.03 | 0.25 | 1 | 16 | 0.5 | 4 | 4 | 4 | 2 | 8 | 16 | 0.5 | 50 | 500 | 125 | 1000 |
| TM425 | AB, CD, EF, YZ, K, HI, LM, UV, WX, Q, RS, O, vpoC | 4 | 1 | 0.25 | 0.03 | 0.25 | 1 | 16 | 0.5 | 4 | 4 | 4 | 2 | 8 | 16 | 0.5 | 50 | 500 | 125 | 1000 |
OXA, oxacillin; CLO, cloxacillin; EM, erythromycin; NFLX, norfloxacin; NOV, novobiocin; TET, tetracycline; KM, kanamycin; CM, chloramphenicol; BC, benzalkonium chloride; CHL, chlorhexidine; EtBr, ethidium bromide; R6G, rhodamine 6G; ACR, acriflavine; TPP; tetraphenylphosphonium chloride; HOE, Hoechst 33342; SDS, sodium dodecyl sulfate; CHO, sodium cholate; DEO, sodium deoxycholate; GLY, sodium glycocholate. MIC values in boldface are smaller than those of the parent strain V. parahaemolyticus AQ3334.
1
Deleted genes: ‘vme’ was omitted.