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Immunity protein release (Im9AF) from oxidized disulfide – containing colE9 constructs. Release of immunity protein following reduction of the disulfide bond is shown as relative fluorescence units (RFU) between Escherichia coli LMG194 (pAG1) cells preincubated with 10 nmol/L of each oxidized colicin E9 construct in the presence (black bars) and absence (white bars) of 2 mmol/L DTT after a 30-min incubation period at 37°C.
