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Disulfide bonds reversibly inhibit cell killing by oxidized Im9-free colE9 constructs. The luminescence induced in Escherichia coli DPD1718 cells preincubated, in duplicate, with 4 nmol/L oxidized colicin E9 constructs, IL1 (○), IL3 (◊), KM1 (Δ), BH29 (□) and 4 nmol/L oxidized and then reduced colE9 constructs, IL1 (•), IL3 (♦), KM1 (▲), BH29 (▪) following addition of 1 mmol/L DTT after 1 min, or with no addition (x) is shown with time.
