Table 2.
In silico analysis of the coverage obtained by the set of primers
| Total number of sequences | 0 mismatch (%) | 1 mismatch (%) | 2 mismatches (%) | |
|---|---|---|---|---|
| Deinococcus-Thermus | 1048 | 89 | 95.7 | 97.2 |
| Marinithermus | 2 | 0 | 0 | 100 |
| Meiothermus | 201 | 88 | 95 | 97 |
| Oceanithermus | 15 | 100 | 100 | 100 |
| Thermus | 318 | 91 | 97 | 97 |
| Vulcanithermus | 3 | 67 | 67 | 100 |
| Deinococcus | 365 | 92 | 98 | 99 |
| Truepera | 132 | 80 | 89 | 92 |
| Proteobacteria | 222,804 | <0.1 | <0.1 | 0.3 |
| Firmicutes | 191,278 | 0 | <0.1 | 25 |
| Bacteroidetes | 79,438 | 0 | 0 | 0.1 |
| Actinobacteria | 46,948 | 0 | 0 | 0.1 |
| Acidobacteria | 13,074 | 0 | 0 | 0.2 |
| Cyanobacteria | 12,752 | 0 | 0 | 0.1 |
| Chloroflexi | 10,437 | 0 | 0.9 | 2.4 |
| Tenericutes | 4234 | 0 | 0 | 7.8 |
| Nitrospirae | 2558 | 0 | <0.1 | 1.6 |
| Fusobacteria | 2345 | 0 | 0 | 0.2 |
| Deferribacteres | 1671 | 0 | 0 | 1.6 |
| Fibrobacteres | 1116 | 0 | 0 | 0.1 |
| Chlorobi | 1101 | 0 | 0 | 3.3 |
| Candidate division OD1 | 998 | 0 | 0 | 0.2 |
| Aquificae | 890 | 0.2 | 0.2 | 0.2 |
| Thermotogae | 770 | 0 | 0 | 0.1 |
| Candidate division TM7 | 758 | 0 | 27.8 | 39.2 |
| Armatimonadetes | 660 | 0 | 0 | 1.4 |
| BD1–5 | 396 | 0 | 7.6 | 69.9 |
| TM6 | 373 | 0 | 0 | 4.6 |
| TA06 | 310 | 0 | 0 | 0.3 |
| Candidate division BRC1 | 265 | 0 | 0 | 0.8 |
| RF3 | 231 | 0 | 0 | 4.3 |
| Candidate division WS6 | 158 | 0 | 0 | 22.2 |
| Candidate division KB1 | 78 | 0 | 0 | 12.8 |
| Spirochetes | 57 | 0 | 0 | 11.4 |
| WCHB1–60 | 51 | 0 | 0 | 84.3 |
| MVP-21 | 24 | 0 | 0 | 37.5 |
| GAL08 | 17 | 0 | 0 | 5.9 |
| Kazan-3B-28 | 13 | 0 | 0 | 15.4 |
The results for Deinococcus-Thermus are detailed according to the genera. The total number of sequences available in the database is indicated for each phylum and genera. The given coverage values correspond to 0, 1, or 2 mismatches between the primers and the 16S rRNA gene sequences.