Table 1.
Plasmids and strains used in this study
| Description | Reference | |
|---|---|---|
| Strain | ||
| GV2260 | Nononcogenic A. tumefaciens strain | Deblaere et al. (1985) |
| MCS | GV2260 containing the pBBR1MCS5 plasmid | Nonaka et al. (2008a,b) |
| acdS | GV2260 containing the pBBRacdS plasmid | Nonaka et al. (2008a,b) |
| virB1acdS | GV2260 containing the pvirB1acdS plasmid | This study |
| virD1acdS | GV2260 containing the pvirD1acdS plasmid | This study |
| virE1acdS | GV2260 containing the pvirE1acdS plasmid | This study |
| Plasmid | ||
| pQEAcdS | Overexpression vector for ACC deaminase; AmpR | This study |
| pBBR1MCS-5 | Broad host-range shuttle vector; GenR | Kovach et al. (1995) |
| pBBRacdS | Overexpression vector for ACC deaminase under the control of the lac promoter; GmR | Nonaka et al. (2008a,b) |
| pvirB1acdS | Overexpression vector for ACC deaminase under the control of the virB1 promoter; GmR | This study |
| pvirD1acdS | Overexpression vector for ACC deaminase under the control of the virD1 promoter; GmR | This study |
| pvirE1acdS | Overexpression vector for ACC deaminase under the control of the virE1 promoter; GmR | This study |
| pIG121-Hm | Binary vector plasmid carrying the b-glucuronidase gene (gusA) between the T-borders; KmR | Ohta et al. (1990) |
| pEKH2 | Binary vector plasmid carrying the b-glucuronidase gene (gusA) between the T-borders; SpR | Hoshikawa et al. (2012) |