Table II.

Temperature of Hydrophobic Exposure (T_h) Values of Antibodies Obtained From Differential Scanning Fluoroscopy Measurements, as a Function of pH

Subclass		Tm (°C)
		pH	4.0	5.0	5.5	6.0	7.0
Mab-A
G1
	Th1		63	68	69.5	70	70.5
	Th2		73	74.5	74.5	NA	NA
G2
	Th1		62.5	68	69.5	70	70.5
	Th2		71.5	73	NA	NA	NA
G4PE
	Th1		56.5	60	61	61.5	62
	Th2		67.5	69.5	70	70	70
Mab-B
G1
Mab-C
G1
	Th1		62.5	67.5	69	69.5	70
	Th2		74	75	75	74.5	74
G2
	Th1		61.5	67	69	70	70.5
	Th2		73.5	74.5	74.5	74.5	NA
G4PE
	Th1		56.5	60	60.5	61	62
	Th2		73	74	74	73.5	73.5
Mab-C
G1
	Th1		63.5	68	69.5	70	70.5
	Th2		76.5	78	78	78	78
G2AAAS
	Th1		58.5	63.5	65	66	66
	Th2		74.5	76.5	77	77.5	77.5
G4
	Th1		59	62.5	64	65	65.5
	Th2		74	75.5	76	76	75.5
G4PE
	Th1		56.5	60.5	61.5	62	62.5
	Th2		74	75.5	75.5	75.5	75.5
Mab-D
G1
	Th1		63	68	69	70	70
	Th2		79	81	81.5	82	82.5
G2AAAS
	Th1		58	63	65	65.5	66
	Th2		75	82.5	82.5	83	83
G4
	Th1		58	62.5	64.5	65.5	65.5
	Th2		78	81	81	81.5	81.5
G4PE
	Th1		56.5	60.5	61.5	62.5	63
	Th2		78.5	81	81.5	81.5	81.5
NA,not available; melting transition was not detected or well defined.

T_m, thermal unfolding midpoint.