Table 3.

Theoretical values and parameter estimates for a pharmacological system described by the operational model of allosterism including constitutive receptor activity (Equation 8)

Parameter	Theoretical (population mean) values	Parameter estimates Mean ± SD
Log(M)	−9	−9.01 ± 0.05
log(εB)	−1	−1.00 ± 0.01
log(α)	−1	−1.00 ± 0.02
log(δ)	−0.30	−0.30 ± 0.01

System- (E_m and χ) and agonist-dependent (K, ε_A) parameters were taken from parameter estimates in Table 2 and kept fixed [Curves from Table 2 (Figure 5A) represent the control curves from those from Table 3 (Figure 5B)]. Two concentrations (10 and 10⁻⁴) were used for the allosteric modulator. Details for Monte Carlo simulated data generation and curve fitting as described at the bottom of Table 2. All parameters were assumed log-normally distributed. The parameter space for exploration with DE was defined as: log(M), (−15,−3); log(ε_B), (−4,0); log(α), (−4,4) and log(δ), (−4,4).