Figure 4.

Post-treatment with indoles leads to T-reg induction, while reciprocally reducing Th17 cells, in vivo. Cells were isolated on Day 15 from inguinal lymph nodes of EAE mice post-treated with vehicle, I3C or DIM. Representative flow cytometry plots show cells gated on CD4 to examine (A) CD4⁺/FoxP3⁺ T-regs or (B) CD4⁺/IL-17⁺ Th17 populations. Data are representative of three independent experiments. Panel C shows total number of T-regs and Th17 cells calculated as described in Figure 3C. (*P < 0.005; EAE + vehicle vs. normal, I3C or DIM; ^#P < 0.05 normal vs. EAE + I3C; ^##P < 0.005; normal vs. EAE + vehicle, EAE + I3C or EAE + DIM).