Figure 6.

Indole treatment leads to generation of T-regs in vivo. Spleen and inguinal LN were isolated from normal mice treated with vehicle, I3C (40 mg·kg⁻¹) or DIM (40 mg·kg⁻¹) daily for 4 days. (A) CD4⁺ gated T cells were examined for CD4⁺/FoxP3⁺ T-regs using flow cytometry. (B) Based on the proportions of T-regs and absolute number of cells isolated from these organs, total cell number of CD4⁺/FoxP3⁺ T-regs were calculated and plotted (*P < 0.05 I3C or DIM vs. naïve). Data presented as mean ± SEM (n = 3 per sample). (C) Expression of FoxP3 in spleen and inguinal LN was measured by RT-PCR, using 18S as an internal control. Splenocytes were isolated from naïve, I3C (40 mg·kg⁻¹) or DIM (40 mg·kg⁻¹) after 4 days daily treatment.