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. 2013 Jun 21;169(6):1352–1371. doi: 10.1111/bph.12226

Figure 6.

Figure 6

Quercetin and allopurinol down-regulate the NLRP3 inflammasome activation to reduce IL-1β in a TXNIP-dependent manner in high glucose (HG)-exposed hepatocyte cells. BRL-3A and HepG2 cells were incubated with HG (30 mM) in the presence or absence of quercetin and allopurinol for the indicated time respectively. Cell culture supernatant was used to measure IL-1β levels by elisa (A) (n = 8), and cellular lysate was used to measure NRLP3, ASC and caspase-1 protein levels by Western blot (B) (n = 4). The data are expressed as the means ± SEM. +++P < 0.001 versus control; *P < 0.05, **P < 0.01, ***P < 0.001 versus HG control. Moreover, BRL-3A and HepG2 cells were transfected with control siRNA or TXNIP siRNA for 48 h and the effect of RNA interference was evaluated by qRT-PCR (C) (n = 4). The data are expressed as the means ± SEM. ***P < 0.001 versus control siRNA. siRNA-treated hepatocyte cells were incubated with HG (30 mM) in the presence or absence of quercetin and allopurinol for the indicated time respectively. Cellular lysate caspase-1 and TXNIP protein levels (D) were measured by Western blot (n = 4) and culture supernatant IL-1β levels (E) were assayed by elisa (n = 8) in siRNA-treated cells. The data are expressed as the means ± SEM. +++P < 0.001 versus control; *P < 0.05, **P < 0.01, ***P < 0.001 versus HG + control siRNA.