TABLE 2.
PCR primers used for amplification of given loci, based on genomic sequence in E. coli K-12
| Locus | MNR type | Size (bp) | MNR length (bp) | Forward primer, 5′-to-3′ | Reverse primer, 5′-to-3′ | Tm (°C) | GenBank accession no. |
|---|---|---|---|---|---|---|---|
| yaiN | Poly(G) | 250 | 10 | AATTTATCCGGTGAATGTGGT | GGACGCCAGAAACACGCTAC | 64 | AY208980 |
| ycgW | Poly(C) | 205 | 8 | GTCAATTACTATCCACTTCA | TTAATTACAGGATGTTCAGTC | 57 | AY208979 |
| b2345 | Poly(C) | 206 | 8 | GTTCTGCTCGTCCTTCTC | GTTGATTGAAATAGATGGTAGC | 58 | AY208974 |
| ykgE | Poly(G) | 203 | 7 | ATTGCATTTGACGTTTGG | AGGGCGTCACCAATACA | 52 | AY208975 |
| osmB | No SSR | 140 | TCACGGAAGTAAGCTCT | CTAAGATGATTCCTGGTTG | 51 | AY208976 | |
| galS | No SSR | 120 | CGCTACATCACGAATGGTG | AAACATCAAGATAACGATCTGG | 59 | AY208977 | |
| pepD | No SSR | 251 | GGAGATAATTGAGACAGTTCAG | ATGTCCCAGGTGACGATG | 56 | AY208978 |