TABLE 4.
Polymorphic site | Locus | Length of sequence (bp) | No. of strains sequenced | MNR + flanking sequenceb
|
Flanking sequence onlyc
|
Microhaplotypes
|
|||
---|---|---|---|---|---|---|---|---|---|
No. of polymorphic sites | Polymorphism level | No. of polymorphic sites | Polymorphism level | No. of microhaplotypes | Polymorphism level | ||||
MNR | yaiN | 198 | 21 | 5 | 0.12 | 2 | 0.05 | 5 | 0.12 |
ycqW | 118 | 21 | 17 | 0.69 | 10 | 0.40 | 10 | 0.40 | |
b2345 | 128 | 26 | 11 | 0.33 | 6 | 0.18 | 11 | 0.33 | |
ykqE | 90 | 25 | 6 | 0.27 | 5 | 0.22 | 6 | 0.27 | |
Mean ± SD | 0.351 ± 0.015 | 0.214 ± 0.005 | 0.280 ± 0.004 | ||||||
Non-SSR | osmB | 140 | 26 | 4 | 0.11 | 4 | 0.11 | 7 | 0.19 |
qalS | 120 | 27 | 1 | 0.03 | 1 | 0.03 | 3 | 0.09 | |
pepD | 251 | 27 | 0 | 0.00 | 0 | 0.00 | 1 | 0.01 | |
Mean ± SD | 0.047 ± 0.001 | 0.047 ± 0.001 | 0.100 ± 0.003 | ||||||
Pd | 0.046 | 0.053 | 0.036 |
Non-SSR loci were sequenced in a 27-strain panel; hence, the comparison to MNR loci was done for the same 27 strains.
Mean number of polymorphic sites when the MNRs are considered as multiple sites.
Mean number of polymorphic sites in sequences flanking the MNR.
One-tailed P value (Student's t test) with unequal variances.