RT-PCR analysis of the presence of transcripts for genes involved in PCP degradation. Left- and right-most lanes, molecular size markers (M); lane 1, wild-type (W) cells grown in the absence (−) of PCP; lane 2, wild-type cells grown in the presence (+) of 50 μM PCP; lanes 3 to 9, mutant strains (as indicated at the bottom) grown in the absence (−) of PCP. pcpA, pcpB, pcpC, and pcpD encode DCHQ dioxygenase, PCP hydroxylase, TCHQ dehalogenase, and TCBQ reductase, respectively.