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. 2013 Nov 9;12:103. doi: 10.1186/1475-2859-12-103

Figure 3.

Figure 3

DHA production by E. coli DH5α cells harboring the plasmid pTrc99A-gldA-nox. Cells were cultivated at 30°C or 37°C for 8 h or 18 h after being induced by IPTG, and collected for biotransformation experiments in 5 mL of reaction buffer with 20 g/L glycerol at 37°C, 200 rpm for 10 h. (A) DHA specific titer; (B) Initial enzymatic activity of GldA and NOX; (C) Initial NAD+/NADH ratio. All data represent the averages ± SDs for three independent samples.