Table 2.
Strains and plasmids used in this study
| Strains or plasmids | Genotype or characteristic | Resources or references |
|---|---|---|
|
E. coli
Strains |
|
|
| DH5α |
F-, φ80d/lacZ∆M15, ∆(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk-, mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1 |
TaKaRa |
| DH10B |
F-,mcrA, ∆(mrr-hsdRMS-mcrBC), φ80lacZ∆M15, ∆lacX74, recA1, endA1, araD139, ∆ (ara, leu)7697, galU, galK, λ-, rpsL, nupG/pMON14272/pMON7124 |
Invitrogen |
| DH1 |
F-, glnV44(AS), λ
-
, rfbC1, gyrA96(NalR), recA1, endA1, thi-1, hsdR17 |
CGSC |
| (No. 6040) | ||
| MG1655 |
F-, λ
-
, rph-1 |
CGSC |
| (No. 6300) | ||
| BW25113 |
rrnB3, ∆lacZ4787, hsdR514, ∆(araBAD)567, ∆(rhaBAD)568 rph-1 |
CGSC |
| (No. 7636) | ||
| Bl21(DE3) |
F–, dcm, ompT, hsdS(rB–, mB–), gal, λ(DE3) |
Novagen |
| YJE005 |
DH5α/pTrc99A-gldA-nox |
This study |
| YJE006 |
DH5α/pTrc99A-gldA-nox + ntt4 |
This study |
|
Plasmids |
|
|
| pMD18-T |
lacZ, pBR322 ori, bla, cloning vector |
TaKaRa |
| pTrc99A |
lacI, pBR322 ori, bla, expression vector |
Amersham Pharmacia |
| pET15K-ntt4 |
ntt4 inserted within NdeI and BamH I sites, kan |
[11] |
| pBCTC-ntt4 |
ntt4 inserted within Sac I and BamH I sites, kan |
This study |
| pBCTD-ntt4 |
ntt4 inserted within Sac I and BamH I sites, kan |
This study |
| pTrc99A-gldA-nox |
gldA and nox transcription under Trc promoter |
This study |
| pTrc99A-gldA-nox + ntt4 | gldA and nox transcription under Trc promoter, ntt4 under gntT105p promoter. | This study |