Table 3.

Primers used in this study ^a

Primer	Sequence(5′-3′)	Function
gldA-F0	TGCTGTATATAGCGCCGCACAAG	gldA_cloning
gldA-R0	AGGTTGGTATTGGCCTGGATTTG
gldA-F1	CAATTTCACACAGGAAACAGACCATGGACCGCATTATTCAATCAC	gldA_amplification
gldA-R1	GTGTATATCTCCTTCTCTAGTAGCGATCTATTATTCCCACTCTTGCAGG
Nox-F1	CTACTAGAGAAGGAGATATACACATGAAAGTCGTAGTCGTAGG	nox_amplification
Nox-R1	CAAAACAGCCAAGCTTGCATGCCTGCAGTTACATATTTTCTAAAGCGGCTTG
gapAP1-F1	TCGGATATCGAGGCGAGTCAGTCGCGTAATGC	Promoter gapAP1 amplification
gapAP1-R1	ACCGAATTCGATCTCATATGTTCCACCAGCTATTTGTTAG
gntT105P-F1	CCGTTGATATCTGAAAGGTGTGCGCGATCTCAC	PromotergntT105P amplification
gntT105P-R1	GGAATTCTATCTCCTTATTCATTTGCGCTGGGTAACGTCAATTT
ntt4-F1	TTCGAGCTCATGAGTAAAACAAACCAGG
ntt4-R1	AGAGGATCCTTAGTGATGATGATGATGATGTTTTTTTATAAAAG	ntt4 amplification
gntT105P-F2	CAAACTCTTTTTGTTTATTTTTCTAAATACATGAAAGGTGTGCGCGATCTC	gntT105P + ntt4 amplification
ntt4-R2	CGTTTTATTTGATGCCTGGATCCGCGTCGACTCTAGAGGATCC
T-F1	GGATCCTCTAGAGTCGACGCGGATCCAGGCATCAAATAAAACG	Terminator BBa_B0015 amplification
T-R1	GTATTTAGAAAAATAAACATATAAACGCAGAAAGGCCCAC

^aThe restriction sites were bolded and the ribosome binding sites (RBS) were underlined.