TABLE 1.
Identification of potential PKCβ substrates in MCP-1-treated monocytes compared to PKCβ-specific antisense ODN treated monocytes.
| Spot number | Protein name | NCBI accession number | Mol. mass (kDa) | Isoelectric point (pI) | Number of peptides (%sequence coverage) |
|---|---|---|---|---|---|
| 1 | Vimentin Tubulin, alpha |
37582 13436317 |
54 50 |
5.0 4.9 |
33 (73%) 3 (11%) |
| 2 | Vimentin ATP synthase, H+ transporting, mitochondrial F1 complex, beta peptide |
37582 16741373 |
54 56 |
5.0 5.2 |
28 (54%) 18 (49%) |
| 3 | Vimentin NF-M protein |
37582 35046 |
54 102 |
5.0 4.9 |
29 (55%) 4 (4%) |
| 4 | Vimentin Tubulin, alpha |
7576229 13436317 |
54 50 |
5.0 4.9 |
46 (73%) 11 (31%) |
| 5 | Lamin B1 | 576840 | 66.6 | 5.1 | 18 (29%) |
| 6 | Lymphocyte cytosolic protein 1 (L-plastin) | 8217500 | 71 | 5.3 | 13 (26%) |
| 7 | Capping protein gelsolin- like | 60655417 | 39 | 5.8 | 14 (34%) |
| 8 | Capping protein gelsolin- like | 60655417 | 39 | 5.8 | 14 (34%) |
| 9 | Annexin I Aflatoxin aldehyde reductase AFAR |
55959292 2736256 |
39 37 |
6.5 6.2 |
17 (47%) 2 (8%) |
| 10 | ENO1 (Enolase 1 variant) | 62896593 | 47 | 7.0 | 25 (53%) |
| 11 | ENO1 (Enolase 1 variant) Lasp-1 protein |
29792061 2135552 |
47 31 |
7.0 6.1 |
6 (18%) 5 (18%) |
| 12 | Bilverdin reductase A Transaldolase 1 |
13543489 14603290 |
34 38 |
6.0 6.3 |
6 (21%) 2 (5%) |