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. Author manuscript; available in PMC: 2014 Oct 15.
Published in final edited form as: Cancer Res. 2013 Oct 4;73(20):10.1158/0008-5472.CAN-13-1558-T. doi: 10.1158/0008-5472.CAN-13-1558-T

Figure 3. IL6 regulates proliferation and survival of PanIN cells.

Figure 3

(A) RT-qPCR for Il6, Il1β, GM-CSF and Slfn4 expression in WT, iKras* and iKras*;IL6−/− mice pancreata. (B) Ki67 immunohistochemistry staining of iKras* and iKras*;IL6−/− mice pancreata 3 weeks, 5 weeks~8 weeks and 17 weeks following pancreatitis. Scale bar 25μm. (C) TUNEL (red) and co-immunofluorescence staining for CK19 (green), SMA (grey) and DAPI (blue). Scale bar 25μm. (D) Ki67 proliferation index in ADM/PanIN, stroma and acinar cells and (E) quantification of apoptotic cells in iKras* and iKras*;IL6−/− mice pancreata. Data represent mean ± SEM, n=3. The statistical difference was determined by two-sided Student’s t-test, comparing the different genotype mice at each time point for each category considered (acini, stroma, ADM/PanIN). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.