Table 8.
Microarray | RT-PCR | |||
---|---|---|---|---|
WKY | SHR | WKY | SHR | |
(A) Control artery relative expression | ||||
Cyba | 393 ± 167 | 1104 ± 169† | 0.928 ± 0.095 | 2.140 ± 0.495† |
(B) Collateral/control ratio | ||||
Agt | 0.159 ± 0.106* | 0.674 ± 0.415 | 0.401 ± 0.088* | 0.563 ± 0.288 |
AGTR1b | 0.463 ± 0.208 | 0.482 ± 0.198* | 3.018 ± 0.703*,† | 0.425 ± 0.156† |
CX3CL1 | 0.564 ± 0.376* | 0.645 ± 0.394 | 0.533 ± 0.102* | 0.479 ± 0.062* |
ESM1 | 3.99 ± 1.298* | 1.81 ± 0.545 | 4.758 ± 1.159* | 2.169 ± 0.356* |
Data are given as relative expression for cyba in control arteries and for collateral/control ratios for the remaining molecules. Results between Mircoarray and RT-PCR were similar except for AGTR1b in WKY where expression was increased rather than decreased, (N ≥ 4; *, †, ‡: P ≤ 0.05 Collateral vs. Control, SHR vs. WKY, qRT-PCR vs. Microarray). Cyba, cytochrome b-245, alpha polypeptide, p22-phox; Agt, angiotensinogen; AGTR1b, angiotensin II receptor, type 1b; CX3CL1, chemokine (C-X3-C motif) ligand 1; ESM1, endothelial cell-specific molecule 1. WKY, Wistar Kyoto; SHR, spontaneously hypertensive rat.