Table 8.

Comparison of microarray and RT-PCR analyses for selected genes important in arterial remodeling

	Microarray		RT-PCR
	WKY	SHR	WKY	SHR
(A) Control artery relative expression
Cyba	393 ± 167	1104 ± 169†	0.928 ± 0.095	2.140 ± 0.495†
(B) Collateral/control ratio
Agt	0.159 ± 0.106*	0.674 ± 0.415	0.401 ± 0.088*	0.563 ± 0.288
AGTR1b	0.463 ± 0.208	0.482 ± 0.198*	3.018 ± 0.703*,†	0.425 ± 0.156†
CX3CL1	0.564 ± 0.376*	0.645 ± 0.394	0.533 ± 0.102*	0.479 ± 0.062*
ESM1	3.99 ± 1.298*	1.81 ± 0.545	4.758 ± 1.159*	2.169 ± 0.356*

Data are given as relative expression for cyba in control arteries and for collateral/control ratios for the remaining molecules. Results between Mircoarray and RT-PCR were similar except for AGTR1b in WKY where expression was increased rather than decreased, (N ≥ 4; *, †, ‡: P ≤ 0.05 Collateral vs. Control, SHR vs. WKY, qRT-PCR vs. Microarray). Cyba, cytochrome b-245, alpha polypeptide, p22-phox; Agt, angiotensinogen; AGTR1b, angiotensin II receptor, type 1b; CX3CL1, chemokine (C-X3-C motif) ligand 1; ESM1, endothelial cell-specific molecule 1. WKY, Wistar Kyoto; SHR, spontaneously hypertensive rat.