Table 1.

Sequences of primer used for biological analysis of rats and pikas hearts obtained by multiple alignments of homologous sequences of at least five different species

Gene		Primer sequence									Predicted PCR product size
18S ribosomal RNA	Forward primer	5′	AGG	CCA	TGA	TTA	AGA	GGG	AC	3′	153 bp
	Reverse primer	5′	TCT	GAT	CGT	CGT	CGA	ACC	TC	3′
β1-adrenoceptor	Forward primer	5′	TGT	GCA	TCA	TGG	CCT	TCG	T	3′	∼392 bp
	Reverse primer	5′	GCA	GTA	GAT	GAT	GGG	GTT	GA	3′
β2-adrenoceptor	Forward primer	5′	TGC	TGA	CCA	AGA	ATA	AGG	CC	3′	∼427 bp
	Reverse primer	5′	AGG	GTG	AAR	GTG	CCC	ATG	AT	3′
Muscarinic m2 receptor	Forward primer	5′	TGT	TCA	GCT	TGG	CCT	GTG	CT	3′	∼250 bp
	Reverse primer	5′	GCA	ATC	ATC	ATR	CCT	GCC	AT	3′
VEGF 189	Forward primer	5′	CCC	ATG	AAG	TGG	TGA	AGT	TC	3′	∼253 bp
	Reverse primer	5′	CTC	ATC	TCT	CCT	ATG	TGC	TG	3′

For VEGF gene sequence was available in database (EU262734).