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. 2013 Oct 29;110(46):18549–18554. doi: 10.1073/pnas.1311628110

Fig. 1.

Fig. 1.

Reduced muscle mass in mice lacking Numb in the Pax7-lineage after injury. (A) TA muscles from Pax7-Numbfl/fl Nbl−/− and Numbfl/fl Nbl−/− adult mice isolated 4 or 14 d after BaCl2 injury and H&E stained. (Scale bars: 50 μm.) (B) CSA of regenerating fibers at 5.5 and 14 d post BaCl2 injury in muscles from Pax7-Numbfl/fl Nbl−/− and Numbfl/fl Nbl−/− littermates. Representative sections of Pax7-Numbfl/fl Nbl−/− and Numbfl/fl Nbl−/− TA muscles at 5.5 dpi stained with antibodies against eMyHC and Laminin (Lam) respectively in B′ and B′′. Nuclei were DAPI stained. (Scale bars: 50 μm.) (C) The mean of the average CSA calculated (n > 3) at 5.5 and 14 dpi. Pax7-Numbfl/fl Nbl−/− showed a statistically significant reduction in regenerating fiber size at both time points. When Numb, but not Nbl, was ablated, a significant reduction in fiber size was observed at 5.5 dpi but not 14 dpi. Data are the mean ± SEM. *P < 0.05; **P < 0.01; ns, not significant.