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. 2013 Oct 29;110(46):18549–18554. doi: 10.1073/pnas.1311628110

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Fig. 4. — Numb-deficient satellite cells have a proliferation deficit. (A) The loss of Numb resulted in a static cell number. Satellite cells were plated in growth medium after treatment with 4OH-T or ethanol; genotypes are as indicated. Triplicate wells were trypsinized daily, stained with 0.4% Trypan Blue, and counted. Data are mean number of cells ± SD (n = 3). (B) Caspase 3/7activity showed that Numb-deficient satellite cells had no increase in apoptosis in culture. ER-Nb^fl/fl Nbl^fl/fl and Nb^fl/fl Nbl^fl/fl satellite cells after 4OH-T or ethanol treatment; data are mean Luciferase levels ± SD (n = 4).