Fig. 6.
Mstn knock-down rescued the proliferation defect in Numb-deficient satellite cells. (A) ER-Nbfl/fl Nblfl/fl satellite cells that were 4OH-T or ethanol treated were transfected with Mstn-specific or scramble siRNAs. Mstn expression was determined by qRT-PCR and Mstn-specific siRNAs knockdown expression by 85%. Data are relative gene expression ± SD (n = 3). (B) Proliferation assays demonstrate that Mstn-specific siRNA rescues the proliferation defect. ER-Nbfl/fl Nblfl/fl satellite cells were 4OH-T or ethanol treated and transfected with Mstn or scramble siRNA. Daily, triplicate wells were counted; data are average cell number ± SD, (n = 3).