Fig. 2.

DIM does not protect MDA-MB-231 xenografts against fractionated radiation. (A) Tumor cells (4 × 10⁶) were injected into each flank of 40 nude mice, and mice were given the indicated treatments. Fractionated irradiation (RT) was carried out so that one flank was exposed while the other flank and the rest of the mouse were shielded. Tumor sizes are means ± SEMs of n = 20 tumors per group. (B) Nude mice containing MDA-MB-231 xenografts were treated without or with one dose of DIM (75 mg/kg) at different times before or after irradiation (20 Gy). Mice were killed at 24 h after irradiation, and tumor extracts were Western blotted for phosphor-ATM or total ATM. (C) Mice were treated ± DIM (75 mg/kg) at the indicated time before or after TBI (20 Gy). Twenty-four hours after TBI, the animals were killed and extracts of kidney tissue were subjected to Western blotting. S1988 in rat ATM corresponds to S1981 in human ATM.