Fig. 1.

Identification of aortic wall self-antigens. Groups of mice were perfused with elastase, then killed immediately (T0, n = 3) or after 30 min (T30, n = 3). Preformed ICs were immunoprecipitated as detailed in Materials and Methods and analyzed by SDS/PAGE under reducing conditions. (A) Bands that were enhanced or appeared de novo (nos.1–5) were excised and submitted for MS analysis. (B) Identity (ID) and molecular weight (MW) of protein bands identified by MS.