Fig. 2.

PISTILLATA (PI, B-class) and AGAMOUS (AG, C-class) gene expression in Rafflesiaceae flowers. (A) Rafflesia PI and AG homologs abbreviated RfPI and RfAG. (B) Sapria, SapPI, and SapAG; In, inner perianth lobes; Out, outer perianth lobes. (C) Rhizanthes, RhPI, and RhAG; PrD, -E, and -F are perianth regions indicated in Fig. S6. Tl, tails. Actin is used as a concentration control. Abbreviations of dissected floral organs are as follows: Br, bracts; Bs, base of the flower; Dia, diaphragm; Dsk, disk; Flr, floor of the floral chamber; Lb, perianth lobes; Ov, ovary; St, stamens; Tb, perianth tube, (see Fig. S4 for the position of these regions). Gene-expression summaries illustrated to the left are shaded as follows: PI expression in yellow, AG expression in dark blue. The overlap of PI and AG in stamens is shown in green and in the proximal lobes of Rhizanthes with blue and yellow hatching.