Fig. 1.
Survey of conformational states adopted by the BmorPBP in delipidated aqueous solution. Shown are 2D [15N,1H]-HMQC correlation NMR spectra at temperature (T) = 20 °C of 0.3 mM solutions of uniformly 15N-labeled BmorPBP containing 50 mM potassium phosphate and 1 mM NaN3 in four different conditions. (A) pH = 8.0. (B) pH = 6.5. (C) Same as B after addition of a saturating amount of bombykol, showing the spectrum of the 1:1 complex with this ligand. (D) Same as C after adjusting the pH to 4.5.