Table 1.

Free energy difference between BmorPBP^A and BmorPBP^B as a function of pH for different BmorPBP–ligand complexes

Ligand	ΔGAB(pH = 0)*	d(ΔGAB)/d(pH)†	n‡
None§	−11.1 ± 0.2	1.5 ± 0.1	1.1 ± 0.1
Hexadecan-1-al	−30.2 ± 2.8	5.3 ± 0.5	4.0 ± 0.4
Hexadecan-1-ol	−28.7 ± 1.1	5.0 ± 0.2	3.7 ± 0.2
Bombykol	−18.9 ± 0.7	3.6 ± 0.1	2.7 ± 0.1

^*ΔG_AB(pH = 0) is the free energy difference between BmorPBP^A and BmorPBP^B extrapolated to pH = 0 in kilocalories per mole.

^†d(ΔG_AB)/d(pH) is the slope obtained from least squares fits of ΔG vs. pH in kilocalories per mole. Least squares r² values ranged from 0.96 to 0.99.

^‡n is the number of titrating groups participating in the conformational transition.

^§Delipidated BmorPBP solution.