Scan rate dependency of normalized thermal unfolding transitions by fluorescence () of A1-1261 (A) and A1-1238 (B) with excitation. Scan rates were (black circles), (open circles), (black squares) and for A1-1261 and for A1-1238 (both with open squares). Insets show representative thermal scans and corresponding baselines before data normalization. Scan rate dependency of thermal unfolding transitions by CD at for A1-1261 (C) and A1-1238 (D). Scan rates were (black circles), (open circles), (black squares), and (open squares).