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Activation of NmeDAH7PS by Different Divalent Metal Ions
| Divalent metal | % Activitya |
|---|---|
| Mn | 100 |
| Cd | 69 |
| Co | 34 |
| Zn | 24 |
| Fe | 15 |
| Cu | 8 |
| Mg | 1 |
| Ni | 0 |
| Ca | 0 |
| Ba | 0 |
| Sr | 0 |
All solutions contained 10 μM EDTA and (except the metal solutions) were treated with Chelex resin before use. The assay mixture contained PEP (180 μM), divalent metal salt (100 μM), and NmeDAH7PS (5 μL, 1.7 mg/mL). Reactions were initiated with the addition of E4P (150 μM).
