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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Cell. 2013 Jul 18;154(3):10.1016/j.cell.2013.06.051. doi: 10.1016/j.cell.2013.06.051

Figure 4. 1q12h copy gain is not stably inherited and requires S phase each cell cycle.

Figure 4

(A) Increased 1q12h copy number in GFP-KDM4A RPE cells is not inherited. FISH of single cell clones derived from RPE KDM4A cells. (B) Average copy gain for 27 single cell clones from A is graphically depicted. (C) Increased copy number of 1q12h requires S phase. (D) 1q12h gain is lost by the end of G2. (E, F) 1q12h copy gain is generated in S phase. Stable GFP-CTRL (E) and GFP-KDM4A (F) RPE cells were arrested in hydroxyurea (HU) for 20 hours and released for the time indicated prior to FISH analysis. Error bars represent the S.E.M. * indicates significant difference from GFP-CTRL (P<0.05) by two-tailed students t-test. For the HU release, P values are based on the comparison of KDM4A to CTRL at each individual time point (F, E, respectively). See also Figure S4.