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Primers used to amplify genes encoding tetracycline resistance in Escherichia coli
| Gene | Forward sequence Primer (5´ to 3´) | Reverse sequence Primer (5´ to 3´) | Annealing Temp (°C) | Product Size (bp) |
|---|---|---|---|---|
|
| ||||
| tetA | GGCGGTCTTCTTCTTCATCATGC | CGGCAGGCAGAGCAAGTAGA | 62 | 501 |
| tetB | CATTAATAGGCCCATCGCTG | TGAAGGTCATCGATAGCAGG | 58 | 929 |
|
| ||||
