Skip to main content
. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Neurobiol Aging. 2013 Sep 20;35(2):10.1016/j.neurobiolaging.2013.08.016. doi: 10.1016/j.neurobiolaging.2013.08.016

Fig. 3. IPAF is involved in IL-1β maturation.

Fig. 3

(A) The mRNA expression of IPAF in astrocytes treated with BSA or PA for 6 or 12hr. The mRNA levels in astrocytes treated with PA and were normalized to astrocytes treated with BSA (n=3). (B, C, D) mRNA and protein levels of silenced IPAF. Astrocytes were cultured with scramble siRNA (scbsi) or siRNA targeting IPAF (IPAF siRNA) for 20hr. The IPAF level was detected by immunoblot and real-time PCR (n=3). (E, F) IL-1β expression levels. Wild-type astrocytes or astrocytes silenced with scramble siRNA or IPAF siRNA or NLRP3 siRNA were cultured for 12hr with astrocyte medium (AM), BSA, or PA. ELISA was performed for IL-1β level and the expression levels of IL-1β were normalized to BSA treated wild-type or silenced astrocytes (n≥3). NA: not available. **: p<0.01. A line indicates comparison between the two bars connected by the line.